Omachi K, Kamura M, Teramoto K, Kojima H, Yokota T, Kaseda S, Kuwazuru J, Fukuda R, Koyama K, Matsuyama S, Motomura K, Shuto T, Suico MA, Kai H.
A split-luciferase-based trimer formation assay as a high-throughput screening platform for therapeutics in Alport syndrome.
Cell Chem Biol.2018 May 17;25(5):634-643.e4. doi:10.1016/j.chembiol.2018.02.003.

Yokota T, Omachi K, Suico MA, Kamura M, Kojima H, Fukuda R, Motomura K, Teramoto K, Kaseda S, Kuwazuru J, Takeo T, Nakagata N, Shuto T, Kai H.
STAT3 inhibition attenuates the progressive phenotypes of Alport syndrome mouse model.
Nephrol Dial Transplant. 2018 Feb 1;33(2):214-223. doi: 10.1093/ndt/gfx246.

Kamei S, Fujikawa H, Nohara H, Ueno-Shuto K, Maruta K, Nakashima R, Kawakami T, Matsumoto C, Sakaguchi Y, Ono T, Suico MA, Boucher RC, Gruenert DC, Takeo T, Nakagata N, Li JD, Kai H, Shuto T.
Zinc Deficiency via a Splice Switch in Zinc Importer ZIP2/SLC39A2 Causes Cystic Fibrosis-Associated MUC5AC Hypersecretion in Airway Epithelial Cells.
EBioMedicine. 2018 Jan;27:304-316. doi: 10.1016/j.ebiom.2017.12.025.

Wakita Y, Sato T, Chosa K, Suico MA, Sasaki R, Kawano S, Hashimoto N, Teranishi Y, Imai Y, Morioka H, Shuto T, Kai H.
Characterization of non-amyloidogenic G101S transthyretin, Biological and Pharmaceutical Bulletin
Biol Pharm Bull. 2018;41(4):628-636. doi: 10.1248/bpb.b17-01021.

Yokota T, Omachi K, Suico MA, Kojima H, Kamura M, Teramoto K, Kaseda S, Kuwazuru J, Shuto T, Kai H.
Bromide supplementation exacerbated the renal dysfunction, injury and fibrosis in a mouse model of Alport syndrome.
PLoS One. 2017 Sep 5;12(9):e0183959. doi: 10.1371/journal.pone.0183959. eCollection 2017.

Koga T, Shiraki N, Yano S, Suico MA, Morino-Koga S, Sato T, Shuto T, Kume S, Kai H.
Mild electrical stimulation with heat shock guides differentiation of embryonic stem cells into Pdx1-expressing cells within the definitive endoderm.
BMC Biotechnol. 2017 Feb 15;17(1):14. doi: 10.1186/s12896-017-0331-z.

Omachi K, Miyakita R, Fukuda R, Kai Y, Suico MA, Yokota T, Kamura M, Shuto T, Kai H.
Long-term treatment with EGFR inhibitor erlotinib attenuates renal inflammatory cytokines but not nephropathy in Alport syndrome mouse model.
Clin Exp Nephrol. 2017 Feb 8. doi: 10.1007/s10157-017-1386-9

Suico MA, Shuto T, Kai H.
Roles and regulations of the ETS transcription factor ELF4/MEF.
J Mol Cell Biol.2017 Jun 1;9(3):168-177. doi: 10.1093/jmcb/mjw051.

Shuto T, Kamei S, Nohara H, Fujikawa H, Tasaki Y, Sugahara T, Ono T, Matsumoto C, Sakaguchi Y, Maruta K, Nakashima R, Kawakami T, Suico MA, Kondo Y, Ishigami A, Takeo T, Tanaka KI, Watanabe H, Nakagata N, Uchimura K, Kitamura K, Li JD, Kai H.
Pharmacological and genetic reappraisals of protease and oxidative stress pathways in a mouse model of obstructive lung diseases.
Sci Rep. 2016 Dec 16;6:39305.

Tasaki Y, Wang WY, Konduru AS, Komatsu K, Matsuyama S, Kai H, Li JD
cAMP-dependent protein kinase A acts as a negative regulator for nontypeable Haemophilus influenzae-induced GM-CSF expression via inhibition of MEK-ERK signaling pathway.
Integr Mol Med. 2016, DOI: 10.15761/IMM.1000261

Kondo T, Goto R, Ono K, Kitano S, Suico MA, Sato M, Igata M, Kawashima J, Motoshima H, Matsumura T, Kai H, Araki E.
Activation of heat shock response to treat obese subjects with type 2 diabetes: a prospective, frequency-escalating, randomized, open-label, triple-arm trial.
Sci Rep. 2016 Oct 19;6:35690.

Suico MA, Taura M, Kudo E, Gotoh K, Shuto T, Okada S, Kai H.
The ETS Factor Myeloid Elf-1-Like Factor (MEF)/Elf4 Is Transcriptionally and Functionally Activated by Hypoxia.
Biol Pharm Bull. 2016;39(4):641-7. doi: 10.1248/bpb.b15-00796.

甲斐 広文
医療機器と薬学
薬剤学2016;76(2):92-98.doi.org/10.14843/jpstj.76.92

Suzuki S, Sargent RG, Illek B, Fischer H, Esmaeili-Shandiz A, Yezzi MJ, Lee A, Yang Y,Kim S, Renz P, Qi Z, Yu J, Muench MO, Beyer AI, Guimaraes AO, Ye L, Chang J, Fine EJ,Cradick TJ, Bao G, Rahdar M, Porteus MH, Shuto T, Kai H, Kan YW, Gruenert DC
TALENs Facilitate Single-step Seamless SDF Correction of F508del CFTR in Airway Epithelial Submucosal Gland Cell-derived CF-iPSCs.
Molecular Therapy Nucleic Acids (2016) 5, e273. doi:10.1038/mtna.2015.43

Fukuda R, Suico MA, Kai Y, Omachi K, Motomura K, Koga T, Komohara Y, Koyama K, Yokota T, Taura M, Shuto T, Kai H.
Podocyte p53 Limits the Severity of Experimental Alport Syndrome.
J Am Soc Nephrol. 2016 Jan;27(1):144-57. doi: 10.1681/ASN.2014111109.

Kai Y, Tsurekawa Y, Fukuda R, Omachi K, Piruzyan M, Okamoto Y, Motomura K, Suico MA, Shuto T, Kondo T, Araki E, Kai H.
The effect of mild electrical stimulation with heat shock on diabetic KKAy mice.
Integr Mol Med. 2015 Dec 17; 3: DOI: 10.15761/IMM.1000190.

首藤恵子，水之江翔太，甲斐広文，首藤 剛.
難治性気道炎症疾患における感染誘導性IL-8産生に対するIL-17Aの作用
アレルギーの臨床 2015 Dec 20; 35(13): 47-52.

Yokoyama T, Takaki S, Chosa K, Sato T, Suico MA, Teranishi Y, Shuto T, Mizuguchi M, Kai H
Structural stabilization of transthyretin by a new compound, 6-benzoyl-2-hydroxy-1H-benzo[de]isoquinoline-1,3(2H)-dione
J Pharmacol Sci. 2015 Dec; 129(4): 240-243.doi:10.1016/j.jphs.2015.09.006

Koga T, Suico MA, Shimasaki S, Watanabe E, Kai Y, Koyama K, Omachi K, Morino-Koga S, Sato T, Shuto T, Mori K, Hino S, Nakao M, Kai H.
Endoplasmic Reticulum (ER) Stress Induces Sirtuin 1 (SIRT1) Expression via the PI3K-Akt-GSK3β Signaling Pathway and Promotes Hepatocellular Injury.
J Biol Chem. 2015 Dec 18; 290(51):30366-74. doi: 10.1074/jbc.M115.664169.

Kadowaki H, Nagai A, Maruyama T, Takami Y, Fitrah PS, Kato H, Honda A, Hatta T, Natsume T, Sato T, Kai H, Ichijo H, Nishitoh H.
Preemptive quality control protects the ER from protein overload via the proximity of ERAD components and SRP.
Cell Rep. 2015 Nov 3; 13(5):944-56. doi: 10.1016/j.celrep.2015.09.047.

甲斐 広文, 近藤 龍也, 荒木 栄一
最適化された微弱パルス電流と温熱の併用療法（physical medicine）の基礎と臨床.
ファルマシア 2015 Nov 1; 51(11):1033-1037

荒木 栄一, 近藤 龍也, 甲斐 広文
細胞内ストレスと生活習慣病-分子シャペロンHsp72の耐糖能改善効果とその機序-
日本体質医学会雑誌 2015 Oct 31; 77(3):163-166.

Susuki-Miyata S, Miyata M, Lee BC, Xu H, Kai H, Yan C, Li JD.
Cross-talk between PKA-Cβ and p65 mediates synergistic induction of PDE4B by roflumilast and NTHi.
Proc Natl Acad Sci U S A. 2015 Apr 7; 112(14):E1800-9. doi: 10.1073/pnas.1418716112.

Masanori Miyata, Ji-Yun Lee, Seiko Susuki-Miyata, Wenzhuo Y. Wang, Haidong Xu, Hirofumi Kai, Koichi S. Kobayashi, Richard A. Flavell & Jian-Dong Li
Glucocorticoids suppress inflammation via the upregulation of negative regulator IRAK-M.
Nature Commun. 2015 Jan 14; 6:6062. doi:10.1038/ncomms7062

Taura M, Kudo E, Kariya R, Goto H, Matsuda K, Hattori S, Vaeteewoottacharn K, McDonald F, Suico MA, Shuto T, Kai H, Okada S.
COMMD1/Murr1 Reinforces HIV-1 Latent Infection through IκB-α Stabilization.
J Virol. 2015 Mar 1; 89(5):2643-58. doi: 10.1128/JVI.03105-14

首藤　剛
上皮型Na+チャネル過剰発現マウスを活用した閉塞性肺疾患治療標的の探索
Jpn J Clin Pharmacol Ther. 2015; 46(1):33-34.

Kariya R, Taura M, Suzu S, Kai H, Katano H, Okada S.
HIV protease inhibitor Lopinavir induces apoptosis of primary effusion lymphoma cells via suppression of NF-κB pathway.
Cancer Lett. 2014 Jan 1;342(1):52-9. doi: 10.1016/j.canlet.2013.08.045.

Kondo T, Ono K, Kitano S, Matsuyama R, Goto R, Suico MA, Kawasaki S, Igata M, Kawashima J, Motoshima H, Matsumura T, Kai H, Araki E
Mild Electrical Stimulation with Heat Shock Reduces Visceral Adiposity and Improves Metabolic Abnormalities in Subjects with Metabolic Syndrome or Type 2 Diabetes: Randomized Crossover Trials
EBioMedicine.2014 Nov;1(1):80-89.doi:10.1016/j.ebiom.2014.11.001

Suico MA, Fukuda R, Miyakita R, Koyama K, Taura M, Shuto T, Kai H.
The transcription factor MEF/Elf4 is dually modulated by p53-MDM2 axis and MEF-MDM2 autoregulatory mechanism.
J Biol Chem. 2014 Sep 19;289(38):26143-54. doi: 10.1074/jbc.M114.580209.

Matsuyama S, Moriuchi M, Suico MA, Yano S, Morino-Koga S, Shuto T, Yamanaka K, Kondo T, Araki E, Kai H.
Mild electrical stimulation increases stress resistance and suppresses fat accumulation via activation of LKB1-AMPK signaling pathway in C. elegans.
PLoS One.2014 Dec 9;9(12):e114690. doi: 10.1371/journal.pone.0114690.

Kondo T, Motoshima H, Igata M, Kawashima J, Matsumura T, Kai H, Araki E.
The role of heat shock response in insulin resistance and diabetes.
Diabetes Metab J. 2014 Apr;38(2):100-6. doi: 10.4093/dmj.2014.38.2.100. Review.

Aritomi K, Ishitsuka Y, Tomishima Y, Shimizu D, Abe N, Shuto T, Irikura M, Kai H, Irie T.
Evaluation of Three-Dimensional Cultured HepG2 Cells in a Nano Culture Plate System: an In Vitro Human Model of Acetaminophen Hepatotoxicity.
J Pharmacpl Sci. 2013; 124: 218-229.

Suzuki S, Shuto T, Sato T, Kaneko M, Takada T, Suico MA, Cyr DM, Suzuki H, Kai H
Inhibition of post-translational N-glycosylation by HRD1 that controls the fate of ABCG5/8 transporter.
Sci Rep. 2014 Mar 3;4:4258. doi: 10.1038/srep04258.

Sargent RG, Suzuki S, Gruenert DC.
Nuclease-Mediated Double-Strand Break (DSB) Enhancement of Small Fragment Homologous Recombination (SFHR) Gene Modification in Human-Induced Pluripotent Stem Cells (hiPSCs).
Methods Mol Biol. 2014;1114:279-90.

Ueno-Shuto K, Kato K, Tasaki Y, Sato M, Sato K, Uchida Y, Sakai H, Ono T, Suico MA, Mitsutake K, Tokutomi N, Kai H, Shuto T.
Lipopolysaccharide decreases single immunoglobulin interleukin-1 receptor-related molecule (SIGIRR) expression by suppressing Sp1 via TLR4-p38 pathway in monocytes and neutrophils.
J Biol Chem. Jun 27;289(26):18097-109. doi:10.1074/jbc.M113.532093jbc.M113.532093.

Taura M, Kariya R, Kudo E, Goto H, Iwawaki T, Amano M, Suico MA, Kai H, Mitsuya H, Okada S.
Comparative analysis of ER stress response into HIV protease inhibitors: lopinavir but not darunavir induces potent ER stress response via ROS/JNK pathway.
Free Radic Biol Med. 2013 Dec;65:778-88. doi: 10.1016/j.freeradbiomed.2013.08.161.

首藤　剛
染時の自然免疫受容体toll-like receptor-2(TLR2)の発現・機能調節とその炎症応答に関する研究
薬学雑誌.2013;133(12):1401-1409

Hiraoka N, Arai Y, Takahashi KA, Mazda O, Kishida T, Honjo K, Tsuchida S, Inoue H, Morino S, Suico MA, Kai H, Kubo T.
Mild electrical stimulation with heat stimulation increase heat shock protein 70 in articular chondrocyte.
J Orthop Res. 2013 Jun;31(6):894-900. doi: 10.1002/jor.22307.

Komatsu K, Lee J, Miyata M, Lim J.H, Jono H, Koga T, Xu H, Yan C, Kai H, Li J.D
Inhibition of PDE4B suppresses inflammation by increasing expression of the deubiquitinase CYLD.
Nat Commun. 2013;4:1684. doi: 10.1038/ncomms2674.

Fukuda R, Suico MA, Koyama K, Omachi K, Kai Y, Matsuyama S, Mitsutake K, Taura M, Morino-Koga S, Shuto T, Kai H.
Mild Electrical Stimulation at 0.1-ms Pulse Width Induces p53 Protein Phosphorylation and G2 Arrest in Human Epithelial Cells.
J Biol Chem. 2013 May 31;288(22):16117-26. doi: 10.1074/jbc.M112.442442.

Sato T, Sako Y, Sho M, Momohara M, Suico MA, Shuto T, Nishitoh H, Okiyoneda T, Kokame K, Kaneko M, Taura M, Miyata M, Chosa K,Koga T, Morino-Koga S, Wada I, Kai H.
STT3B-Dependent Posttranslational N-Glycosylation as a Surveillance System for Secretory Protein.
Mol Cell.2012 Jul 13;47(1):99-110. Epub 2012 May 17.

Komori H, Nishi K, Uehara N, Watanabe H, Shuto T, Suenaga A, Maruyama T, Otagiri M.
Characterization of hepatic cellular uptake of α1-acid glycoprotein (AGP), part 2: involvement of hemoglobin β-chain on plasma membranes in the uptake of human AGP by liver parenchymal cells. J. Pharm. Sci. 101(4):1607-15 (2012)

Komori H, Watanabe H, Shuto T, Kodama A, Maeda H, Watanabe K, Kai H, Otagiri M, Maruyama T.
α1-acid glycoprotein up-regulates CD163 via TLR4/CD14 pathway: possible protection against hemolysis-induced oxidative stress.
J Biol Chem. 2012 Aug.31.

Lee J, Komatsu K, Lee BC, Lim JH, Jono H, Xu H, Kai H, Zhang ZJ, Yan C, Li JD
Phosphodiesterase 4B Mediates Extracellular Signal-regulated Kinase-dependent Up-regulation of Mucin MUC5AC Protein by Streptococcus pneumoniae by Inhibiting cAMP-protein Kinase A-dependent MKP-1 Phosphatase Pathway.
J Biol Chem. 2012 Jun 29;287(27):22799-811. Epub 2012 May 18.

Morino-Koga S, Yano S, Kondo T, Shimauchi Y, Matsuyama S, Okamoto Y, Suico MA, Koga T, Sata T, Shuto T, Arima H, Wada I, Araki E, Kai H.
Insulin receptor activation through its accumulation in lipid rafts by mild electrical stress.
J Cell Physiol. 2012 Jun 27. doi: 10.1002/jcp.24149. [Epub ahead of print]

首藤 剛
新規ダニ誘発性掻痒自然発症モデルマウスの病態解析．
生化学．84(5)，352-355，2012

Matsuno T, Kariya R, Yano S, Morino-Koga S, Taura M, Suico MA, Shimauchi Y, Matsuyama S, Okamoto Y, Shuto T, Kai H, Okada S.
Diethyldithiocarbamate induces apoptosis in HHV-8-infected primary effusion lymphoma cells via inhibition of the NF-κB pathway.
Int J Oncol. 2012 Apr;40(4):1071-8.

Mizunoe S, Shuto T, Suzuki S, Matsumoto C, Watanabe K, Ueno-Shuto K, Suico MA, Onuki K, Gruenert DC, Kai H.
Synergism Between Interleukin (IL)-17 and Toll-like Receptor 2 and 4 Signals to Induce IL-8 Expression in Cystic Fibrosis Airway Epithelial Cells.
J Pharmacol Sci. 2012 Apr;118(4):512-20. Epub 2012 Mar 29.

Koga T, Kai Y, Fukuda R, Morino-Koga S, Suico MA, Koyama K, Sato T, Shuto T, Kai H.
Mild electrical stimulation and heat shock ameliorates progressive proteinuria and renal inflammation in mouse model of Alport syndrome.
PLoS One 7(8):e43852.(2012)

Kondo T, Sasaki K, Matsuyama R, Morino-Koga S, Adachi H, Suico MA, Kawashima J, Motoshima H, Furukawa N, Kai H, Araki E.
Hyperthermia With Mild Electrical Stimulation Protects Pancreatic β-Cells From Cell Stresses and Apoptosis.
Diabetes. 2012 Apr;61(4):838-47. Epub 2012 Feb 23.

Taura M, Suico MA, Koyama K, Komatsu K, Miyakita R, Matsumoto C, Kudo E, Kariya R, Goto H, Kitajima S, Takahashi C, Shuto T, Nakao M, Okada S, Kai H.
Rb/E2F1 Regulates the Innate Immune Receptor Toll-Like Receptor 3 in Epithelial Cells.
Mol Cell Biol. 2012 Apr;32(8):1581-90. Epub 2012 Feb 6.

Watanabe K, Shuto T, Sato M, Onuki K, Mizunoe S, Suzuki S, Sato T, Koga T, Suico MA, Kai H, Ikeda T.
Lucidenic acids-rich extract from antlered form of Ganoderma lucidum enhances TNFα induction in THP-1 monocytic cells possibly via its modulation of MAP kinases p38 and JNK.
Biochem Biophys Res Commun. 2011 Apr 29;408(1):18-24.

Hashimoto Y, Shuto T, Mizunoe S, Tomita A, Koga T, Sato T, Takeya M, Suico MA, Niibori A, Sugahara T, Shimasaki S, Sugiyama T, Scholte B, Kai H.
CFTR-deficiency renders mice highly susceptible to cutaneous symptoms during mite infestation.
Lab Invest. 2011 Apr;91(4):509-18.

Sugiyama T, Shuto T, Suzuki S, Sato T, Koga T, Suico MA, Kusuhara H, Sugiyama Y, Cyr DM, Kai H.
Posttranslational negative regulation of glycosylated and non-glycosylated BCRP expression by Derlin-1.
Biochem Biophys Res Commun. 2011 Jan 21;404(3):853-8.

Yano S, Morino-Koga S, Kondo T, Suico MA, Koga T, Shimauchi Y, Matsuyama S, Shuto T, Sato T, Araki E, Kai H.
Glucose Uptake in Rat Skeletal Muscle L6 Cells Is Increased by Low-Intensity Electrical Current Through the Activation of the Phosphatidylinositol-3-OH kinase (PI-3K) / Akt Pathway.
J Pharmacol Sci. 2011 Jan;115(1):94-8. Epub 2010 Dec 18.;115(1).

Taura M, Suico MA, Fukuda R, Koga T, Shuto T, Sato T, Morino-Koga S, Okada S, Kai H.
MEF/ELF4 transactivation by E2F1 is inhibited by p53.
Nucleic Acids Res. 2011 Jan 1;39(1):76-88.

Shimasaki S, Koga T, Shuto T, Suico MA, Sato T, Watanabe K, Morino-Koga S, Taura M, Okada S, Mori K, Kai H.
Endoplasmic reticulum stress increases the expression and function of toll-like receptor-2 in epithelial cells.
Biochem Biophys Res Commun. 2010 Nov 12;402(2):235-40.

Adachi H, Kondo T, Ogawa R, Sasaki K, Morino-Koga S, Sakakida M, Kawashima J, Motoshima H, Furukawa N, Tsuruzoe K, Miyamura N, Kai H, Araki E.
An acylic polyisoprenoid derivative, geranylgeranylacetone protects against visceral adiposity and insulin resistance in high-fat-fed mice.
Am J Physiol Endocrinol Metab. 2010 Nov;299(5):E764-71.

Ota K, Fujimori H, Ueda M, Jono H, Shinriki S, Ota T, Sueyoshi T, Taura M, Taguma A, Kai H, Shinohara M, Ando Y.
Midkine expression is correlated with an adverse prognosis and is down-regulated by p53 in oral squamous cell carcinoma.
Int J Oncol. 2010 Oct;37(4):797-804.

Shuto T, Ono T, Ohira Y, Shimasaki S, Mizunoe S, Watanabe K, Suico MA, Koga T, Sato T, Morino S, Sato K, Kai H.
Curcumin decreases toll-like receptor-2 gene expression and function in human monocytes and neutrophils.
Biochem Biophys Res Commun. 2010 Aug 6;398(4):647-52.

Oba M, Suico MA, Morino S, Yano S, Matsuno T, Koga T, Sato T, Shuto T, Kai H.
Modified mild heat shock modality attenuates hepatic ischemia/reperfusion injury.
J Surg Res. 2010 Aug;162(2):213-20.

Miyata M, Sato T, Kugimiya M, Sho M, Nakamura T, Ikemizu S, Chirifu M,
Mizuguchi M, Nabeshima Y, Suwa Y, Morioka H, Arimori T, Suico MA, Shuto T,
Sako Y, Momohara M, Koga T, Morino-Koga S, Yamagata Y, Kai H.
Crystal structure of green tea polyphenol(-)-epigallocatechin gallate
(EGCG)-transthyretin complex reveals novel binding site distinct from
thyroxine binding site.
Biochemistry 2010 Jul 27;49(29):6104-14.

Taura M, Fukuda R, Suico MA, Eguma A, Koga T, Shuto T, Sato T, Morino-Koga
S, Kai H.
TLR3 induction by anticancer drugs potentiates poly I:C-induced tumor cell
apoptosis.
Cancer Sci. 2010 Jul;101(7):1610-17.

Yoshida H, Takamura N, Shuto T, Ogata K, Tokunaga J, Kawai K, Kai H.
The citrus flavonoids hesperetin and naringenin block the lipolytic actions
of TNF-alpha in mouse adipocytes.
Biochem Biophys Res Commun. 2010 Apr 9;394(3):728-32.

Miyata M, Sato T, Mizuguchi M, Nakamura T, Ikemizu S, Nabeshima Y, Susuki S,
Suwa Y, Morioka H, Ando Y, Suico MA, Shuto T, Koga T, Yamagata Y, Kai H.
Role of the glutamic acid 54 residue in transthyretin stability and
thyroxine binding. Biochemistry. 2010 Jan 12;49(1):114-23.

Kato K, Lillehoj EP, Kai H, Kim KC.
MUC1 expression by human airway epithelial cells mediates pseudomonas
aeruginosa adhesion.
Front Biosci (Elite Ed). 2010 Jan 1;2:68-77.

近藤龍也、佐々木一成、古賀（森野）沙緒里、松山利奈、甲斐広文、荒木栄一
温熱電流同時印加療法、
BIO Clinica、25（２）、78-83　（2010）

Susuki S, Sato T, Miyata M, Momohara M, Suico MA, Shuto T, Ando Y, Kai H.
The endoplasmic reticulum-associated degradation of transthyretin variants is negatively regulated by BiP in mammalian cells.
J Biol Chem. Feb 2.in press (2009)

Suico, M.A., Tanaka, A., Shuto, T., and Kai, H. (2009).
The expression of antimicrobial peptide lysozyme is increased by treatment with silver nanoparticle (Atomyball S) in mammalian epithelial cells.
Journal of Health Science 55, 456-462.

Sugahara, T., Koga, T., Ueno-Shuto, K., Shuto, T., Watanabe, E., Maekawa, A., Kitamura, K., Tomita, K., Mizuno, A., Sato, T., et al. (2009).
Calreticulin positively regulates the expression and function of epithelial sodium channel.
Exp Cell Res 315, 3294-3300.

Oba, M., Suico, M.A., Morino, S., Yano, S., Matsuno, T., Koga, T., Sato, T., Shuto, T., and Kai, H. (2009).
Modified Mild Heat Shock Modality Attenuates Hepatic Ischemia/Reperfusion Injury.
J Surg Res.

Miyata, M., Sato, T., Mizuguchi, M., Nakamura, T., Ikemizu, S., Nabeshima, Y., Susuki, S., Suwa, Y., Morioka, H., Ando, Y., et al. (2009).
The role of Glu54 residue in transthyretin stability and thyroxine binding.
Biochemistry.

Kondo, T., Sasaki, K., Adachi, H., Nakayama, Y., Hatemura, M., Matsuyama, R., Tsuruzoe, K., Furukawa, N., Motoshima, H., Morino, S., et al. (2009).
Heat shock treatment with mild electrical stimulation safely reduced inflammatory markers in healthy male subjects.
Obesity Research & Clinical Practice in press

Kai H, Suico MA, Morino S, Kondo T, Oba M, Noguchi M, Shuto T, Araki E.
A novel combination of mild electrical stimulation and hyperthermia: general
concepts and applications.
Int J Hyperthermia. 2009 Dec;25(8):655-60.

Lim JH, Kim HJ, Komatsu K, Ha U, Huang Y, Jono H, Kweon SM, Lee J, Xu X,
Zhang GS, Shen H, Kai H, Zhang W, Xu H, Li JD.
Differential regulation of Streptococcus pneumoniae-induced human MUC5AC mucin expression through distinct MAPK pathways.
Am J Transl Res. 2009 May 8;1(3):300-11.

Sunao Katsuki, Kazunori Mitsutake, Masahiko Yano, Hidenori Akiyama, Hirofumi Kai and Tsuyoshi Shuto
Non-thermal and Transient Thermal Effects of Burst 100 MHz Sinusoidal
Electric Fields on Apoptotic Activity in HeLa Cells, IEEE Trns. Dielectr.
Electr. Insul. In press

荒木栄一、近藤龍也、西川武志、森野沙緒里、甲斐広文
細胞内ストレスを標的とした糖尿病治療
日本体質医学会雑誌71巻３号30-34（2009）

甲斐広文、森野沙緒里、近藤龍也、荒木栄一
温熱療法と微弱パルス電流療法
アンチ●エイジング医学―日本抗加齢医学会雑誌、５巻、３号、36-42　（2009）

Yasuaki Hashimoto, Tsukasa Okiyoneda, Kazutsune Harada, Keiko Ueno,Takuya Sugahara, Atsushi Yamashita, Tsuyoshi Shuto, Mary Ann Suico,and Hirofumi Kai Phosphatidic acid metabolism regulate the intracellular trafficking and retrotranslocation of cystic fibrosis transmembrane conductance regulator.BBA-Mol Cell Res. 2008, 1783(1), 153-162

Susuki S., Ando Y., Sato T., Nishiyama M., Miyata M., Suico MA., Shuto T., Kai H., Multi-elemental analysis of serum and amyloid fibrils in familial amyloid polyneuropathy patients. Amyloid (The Journal of Protein Folding Disorders) 15(2):108-116, 2008

Koga T., Lim J.H., Jono H., Ha U.H., Xu H., Ishinaga H., Morino S., Xu X., Yan C., Kai H., Li J.D., Tumor suppressor cylindromatosis (CYLD) acts as a negative regulator for Streptococcus pneumoniae-induced NFAT signaling. J. Biol. Chem. 283(18):12546-54,2008

Oba M., Yano S., Shuto T., Suico M.A., Eguma A., and Kai H. Interferon-gamma down-regulated Hsp27 and enhances hyperthermia-induced tumor cell death in vitro and tumor suppression in vivo. Int. J. Oncol. 32:1317-1324, 2008

Suwa Y., Nakamura T., Toma S., Ikemizu S., Kai H., Yamagata Y., Preparation, crystallization and preliminary X-ray diffraction analysis of the DNA-binding domain of the Ets transcription factor in complex with target DNA. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64(Pt 3):171-4, 2008.

Hidaka M., Nagata M., Kawano Y., Sekiya H., Kai H., Yamasaki K., Okumura M., Arimori K., Inhibitory effects of fruit juices on cytochrome P450 2C9 activity in vitro. Biosci. Biotechnol. Biochem. 72(2):406-11, 2008.

Ueno K., Koga T., Kato K., Golenbock D.T., Gendler S.J., Kai H., and KimK.C., MUC1 mucin is a negative regulator of toll-like receptor signaling.Am. J. Respir. Cell Mol. Biol. 38(3):263-268, 2008.

Iwakiri T., Okumura M., Matsunaga N., Ichihara E., Shiotsuki S., Nagata M.,Kumagai Y., Kai H., and Arimori K., Hepatocyte growth factor increasesuptake of estradiol 17beta-d-glucuronide and Oatp1 protein level in rathepatocytes. Eur. J. Pharmacol. 580: 19-26, 2008.

Okiyoneda T.,. Niibori A., Harada K.,. Kohno T., Michalak M., Duszyk M., Wada I., Ikawa M.,. Shuto T., Suico M.A,, Kai H. Role of calnexin in the ER quality control and productive folding of CFTR; differential effect of calnexin knockout on wild-type and DF508 CFTR.  BBA - Molecular Cell Research,1783:1585-94, 2008

Takashi Furuta, Tsuyoshi Shuto, Shogo Shimasaki, Yuko Ohira, Mary AnnSuico, Dieter C Gruenert and Hirofumi Kai. DNA demethylation-dependent enhancement of toll-like receptor-2 gene expression in cystic fibrosis epithelial cells involves SP1-activated transcription. BMC Molecular Biology. Apr 21;9:39, 2008

Seo T, Ishitsu T, Oniki K, Abe T, Shuto T, Nakagawa K, ABCC2 haplotype is not associated with drug-resistant epilepsy but with mental retardation. J Pharm Pharmacol 60, 631-635, 2008

Manabu Taura, Ayaka Eguma, Mary Ann Suico, Tsuyoshi Shuto, Tomoaki
Koga,Kensei Komatsu, Takefumi Komune, Takashi Sato, Hideyuki Saya, Jian-Dong
Li and Hirofumi Kai, p53 regulates TLR3 expression and function in human
epithelial cell lines. Mol. Cell. Biol. 28(21):6557-67, 2008

Jaemi Kim, Yoshihiro Motomiya, Mitsuharu Ueda, Masaaki Nakamura, Yohei
Misumi, Shiori Saito, Shinji Ikemizu, Shogo Misumi, Kazutoshi Ota, Satoru
Shinriki, Hirofumi Kai and Yukio Ando, Role of conformational change in the
C-terminus of β2-microglobulin in dialysis-related amyloidosis. Ann. Clin.
Biochem. 45:489-495,2008.

Saori Morino, Mary Ann Suico, Tatsuya Kondo, Erika Sekimoto, Shuichiro Yano. Tomoko Matsuda, Takashi Matsuno, Tsuyoshi Shuto, Eiichi Araki, and Hirohumi Kai. Mild Electrical Stimulation Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degredation. J Pharmacol Sci. 108,222-226 (2008)

Kensei Komatsu, Hirofumi Jono, Jae Hyang Lim, Akira Imasato, Haidong Xu, Hirofumi Kai, Chen Yan and Jian-Dong Li, Glucocorticoids inhibit nontypeable Haemophilus influenzae-induced MUC5AC mucin expression via MAPK phosphatase-1-dependent inhibition of p38 MAPK. Biochem. Biophys. Res. Commun.377(3):763-8(2008)

Toru Murakami, Hideaki Harada, Mary Ann Suico, Tsuyoshi Shuto, Shinya Suzu, Hirofumi Kai, and Seiji Okada, Ephedrae Herba, a Component of Japanese Herbal Medicine Mao-to, Efficiently Activates the Replication of Latent Human Immunodeficiency Virus Type 1 (HIV-1) in a Monocytic Cell Line. Biol. Pharm. Bull. 31(12)2334-2337(2008)

Saori Morino, Tatsuya Kondo, Kazunari Sasaki, Hironori Adachi, Mary Ann Suico, Erika Sekimoto, Tomoko Matsuda, Tsuyoshi Shuto, Eiichi Araki, and Hirofumi Kai. Mild Electrical Stimulation with heat shock ameliorates insulin resistance via enhanced insulin signaling. Increases Ubiquitinated Proteins and Hsp72 in A549 Cells via Attenuation of Proteasomal Degredation. PLoS ONE 3(12):e4068 (2008)

Takashi Sato, Seiko Susuki, Masanori Miyata, Yukio Ando, Tsuyoshi Shuto, Mary Ann Suico and Hirofumi Kai, Inhibition of intracellular tetramerization induces the proteasomal degradation of amyloidogenic transthyretin variants.XIth International Symposium on Amyloidosis (Edited by M.Skinner, J.L. Berk, L.H. Connors, D.C. Seldin) CRC Press pp188-190 (2008)

甲斐広文、森野沙緒里、近藤龍也、荒木栄一、微弱パルス電流の新たな生物活性とその治療的応用、「化学工業」Vol.59 No.12, 57-61(2008)

村上　徹、首藤　剛、甲斐広文、岡田誠治、竹内　晃　NK細胞に対する温熱の影響　「がんの温熱免疫療法―ハイパーサーミック●イムノロジーー」吉川敏一監修、診断と治療社（株）pp38-42 (2008)

Kazutsune Harada ,Tsukasa Okiyoneda,Yasuaki Hashimoto,Kimiko Oyokawa, Kimitoshi Nakamura,Mary Ann Suico,Tsuyoshi Shuto, Hirofumi Kai.
Curucumin enhances cystic fibrosis transmembrane regulator expression by down-regulating calreticuline, Biochem. Biophys. Res. Commun. 353, 351-356 (2007)

Takashi Sato, Seiko Susuki, Mary Ann Suico, Masanori Miyata, Yukio Ando, Mineyuki Mizuguchi, Makoto Takeuchi, Mizuki Dobashi, Tsuyoshi Shuto, Hirofumi Kai, Endoplasmic reticulum quality control regulates the fate of transthyretin variants in the cell. The EMBO Journal.26.2501-2512 (2007)

Tsuyoshi Shuto, Takashi Furuta, Judy Cheung, Dieter C. Gruenert, Yuko Ohira, Shogo Shimasaki, Mary Ann Suico, Keizo Sato, and Hirofumi Kai
Increased responsiveness to TLR2 and TLR4 ligands during dimethylsulfoxide-induced neutrophil-like differentiation of HL-60 myeloid leukemia cells. Leukemia Research. 31(12).1721-8(2007)

Chirifu M, Hayashi C, Nakamura T, Toma S, Shuto T, Kai H, Yamagata Y, DavisSJ, Ikemizu S. Crystal structure of the IL-15-IL-15Ralpha complex, acytokine-receptor unit presented in trans. Nature Immunology.8(9):1001-7 (2007)

Kato K, Lu W, Kai H, Kim KC. Phosphoinositide 3-kinase is activated by MUC1but not responsible for MUC1-induced suppression of TLR5 signaling. Am JPhysiol Lung Cell Mol Physiol. 293(3):L686-92(2007)

Sakai A, Koga T, Lim JH, Jono H, Harada K, Szymanski E, Xu H, Kai H, Li JD.The bacterium, nontypeable Haemophilus influenzae, enhances host antiviralresponse by inducing Toll-like receptor 7 expression. Evidence for negativeregulation of host anti-viral response by CYLD. FEBS Journal, 274, 3655-3668(2007)

Huang Y, Mikami F, Jono H, Zhang W, Weng X, Koga T, Xu H, Yan C, Kai H, LiJD. Opposing roles of PAK2 and PAK4 in synergistic induction of MUC5ACmucin by bacterium NTHi and EGF. Biochem Biophys Res Commun. 359, 691-696(2007).

Hideki Harada, Toru Murakami, Seow Shi Tea, Akira Takeuchi, Tomoaki Koga,  Seiji Okada, Mary Ann Suico, Tsuyoshi Shuto, Hirofumi Kai, Heat shock suppresses human NK cell cytotoxicity via regulation of perforin International Journal Of Hyperthermia .23:8,657-665(2007)

橋本 泰明
「ドパミン神経機能維持と神経毒性保護効果を併せ持つ
抗パーキンソン病タンパク質の発見！！」
ファルマシア「環境・衛生」vol.43 No.12 2007 1232-1234

Mikami F, Lim JH, Ishinaga H, Ha UH, Gu H, Koga T, Jono H, Kai H, Li JD. TGF-beta -Smad3/4 signaling pathway acts as a positive regulator for TLR2 induction by bacteria via a dual-mechanism involving functional cooperation with NF-kbeta and MAPK phosphatase 1-dependent negative cross-talk with p38 MAPK.　 J Biol Chem. 281, 22397-22408, 2006.(2006)

Tsukasa Okiyoneda, Taijun Kono, Akiko Niibori, Kazutsune Harada, Hiroyuki Kusuhara, Tappei Takada, Tsuyoshi Shuto, Mary Ann Suico, Yuichi Sugiyama, Hirofumi Kai, Calreticulin Facilitates the Cell Surface Expression of ABCG5/G8, Biochem. Biophys. Res. Commun. 347, 67-75(2006)

Kazutsune Harada ,Tsukasa Okiyoneda,Yasuaki Hashimoto,Keiko Ueno,
Kimitoshi Nakamura,Kaori Yamahira Takuya Sugahara,Tsuyoshi Shuto,
Ikuo Wada,Mary Ann Suico and Hirofumi Kai.
Calreticulin negatively regulates the cell surface expression of CFTR.
J Biol Chem. 281: 12841-12848(2006)

Hidaka M, Okumura M, Ogikubo T, Kai H, Fujita KI, Iwakiri T, Yamasaki K, Setoguchi N, Matsunaga N, Arimori K. Transient inhibition of CYP3A in rats by star fruit juice. Drug Metab Dispos.;34(3):343-5 (2006)

Shuto, T., Furuta, T., Oba, M., Xu, H., Li, JD., Cheung, J., Gruenert, DC., Uehara, A., Suico, MA., Okiyoneda, T., and Kai, H. Promoter hypomethylation of toll-like receptor-2 gene is associated with increased proinflammatory response towards bacterial peptidoglycan in cystic fibrosis bronchial epithelial cells. FASEB J.. 20: 782-784, (2006)

Sato, T., Ando, Y., Susuki, S., Mikami, F., Ikemizu, S., Nakamura, M., Suhr, O., Anraku, M., Kai, T., Suico, MA., Shuto, T., Mizuguchi, M., Yamagata, Y., and Kai, H. Chromium (III) ion and thyroxine cooperate to stabilize the transthyretin tetramer and suppress in vitro amyloid fibril formation. FEBS letter., ;580(2):491-6.(2006)

Suico M.A., Nakamura H., Lu Z., Saito H., Shuto T., Nakao M. and Kai H. SUMO down-regulates the activity of Elf4/Myeloid Elf-1-like factor. Biochem. Biophys. Res. Commun. 348: 880-888,(2006)

Okiyoneda T., Niibori A., Harada K., Kono T., Kusuhara H., Takada T., Shuto T., Suico M.A., Sugiyama Y. and Kai H.Bafilomycin A1-Sensitive Pathway is required for the Maturation of Cystic Fibrosis Transmembrane Conductance Regulator. Biochem. Biochim. Acta(Mol. Cell Res.)in press,(2006)

橋本 泰明，沖米田 司，甲斐 広文
「RIの逆襲」チャネル研究におけるアイソトープの活用
～125Iを用いたクロライドチャネルCFTRのチャネル活性評価法～
細胞工学　「RIの逆襲」vol.25 No.9 2006 1082-1088

Yoshida H, Jono H, Kai H, Li JD. The tumor suppressor CYLD acts as a negative regulator for toll-like receptor 2 signaling via negative cross-talk with TRAF6 and TRAF7. J Biol Chem., 2005 Dec 9;280(49):41111-21. Epub 2005 Oct 17.

Shuto, T., Kato, K., Mori, Y., Viriyakosol, S., Oba, M., Furuta, T., Okiyoneda,
T., Arima, H., Suico, M. A., and Kai, H. Membrane-anchored CD14 is required for
LPS-induced TLR4 endocytosis in TLR4/MD-2/CD14 overexpressing CHO cells.
Biochemical & Biophysical Research Communications, 2005 Dec 23;338(3):1402-9. Epub 2005 Oct 26.

T Koga, H Harada, SS Tea, S Okada, MA Suico, T Shuto and H Kai, Hyperthermia suppresses the cytotoxicity of NK cells via down-regulation of perforin/granzyme B expression., Biochem Biophys Res Commun. 2005 Dec 2;337(4):1319-23.

Mikami F, Gu H, Jono H, Andalibi A, Kai H, Li JD. Epidermal growth factor receptor acts as a negative regulator for bacterium nontypeable Haemophilus influenzae-induced toll-like receptor 2 expression via a Src-dependent p38 MAP kinase signaling pathway. J Biol Chem. 2005 Oct 28;280(43):36185-94.

Shuji Shimohara, Toru Murakami, Mari Morikawa, Junko Matsuo, Shin- ichi　Nagayama, Tsuyoshi Shuto, Mary Ann Suico, Tsukasa Okiyoneda,
Isao Yamatsu, Toru Mizushima, Tatsuya Shimasaki and Hirofumi Kai,
Vitamins K1 and K2 potentiate hyperthermia by down regulating Hsp72
expression in vitro and in vivo. International Journal of Oncology. Int J Oncol. 2005 Dec;27(6):1527-33.

原田一恒, 沖米田司, 首藤　剛, 甲斐広文. 小胞体分子シャペロンカルレティキュリンによるCFTR細胞内輸送制御機構のsiRNAを用いた解析,炎症と免疫, vol.13 no.1, 19-25 (2005)

T. Roger, I. Miconnet, A.-L. Schiesser, H. Kai, K. Miyake, T. Calandra. Critical role for Ets, AP-1 and GATA-like transcription factors in regulating mouse Toll-like receptor 4 (Tlr4) gene expression, Biochem. J. 387, 355-365 (2005)

T. Koga, M.A. Suico, H. Nakmura, M. Taura, Z. Lu, T. Shuto, T. Okiyoneda, H. Kai. Sp1-dependent regulation of Myeloid Elf-1 like factor in human epithelial cells. FEBS letter. 579 (13), 2811-2816 (2005)

Mary Ann Suico, Tomoaki Koga, Tsuyoshi Shuto, Akinori Hisatsune, Zhuo Lu,
Carol Basbaum, Tsukasa Okiyoneda, Hirofumi Kai，Sp1 is involved in the transcriptional activation of lysozyme in epithelial cells，Biochem.Biophys.Res.Chem. 324,1302-1308(2004)

T. Okiyoneda, K. Harada, K. Yamahira, I. Wada, Y. Hashimoto, K. Ueno, M. A.Suico, T. Shuto. and H. Kai, Characterization of the trafficking pathway of cystic fibrosis transmembrane conductance regulator in baby hamster kidney cells. J. Pharmacol. Sci. 95,471-475 (2004)

M. Suico, Z. Lu, T. Shuto, T. Koga, T. Uchikawa, H. Yoshida, K. Matsuzaki, M. Nakao, J.-D. Li, and H. Kai, The regulation of human β-defensin 2 by the ETS transcription factor MEF is enhanced by PML protein, J. Pharmacol. Sci. 95,466-470 (2004)

Zhuo Lu, Kyoung-Ah Kim, Mary Ann Suico, Tsuyoshi Shuto, Jian-Dong Li and Hirofumi Kai, MEF up-regulates human beta-defensin 2 expression in epithelial cells
FEBS Letters, 561, 117-121 (2004)

Mary Ann Suico, Hiroki Yoshida, Yoshiyuki Seki, Tomoko Uchikawa, Zhuo Lu,Tsuyoshi Shuto, Kazuhito Matsuzaki, Mitsuyoshi Nakao, Jian-Dong Li, and Hirofumi Kai, The ETS transcription factor MEF up-regulates lysozyme transcription in Epithelial cells through interaction with PML protein J.Biol.Chem. 279: 19091-19098 (2004)

Tsukasa Okiyoneda, Kazutsune Harada, Motohiro Takeya, Kaori Yamahira, IkuoWada, Tsuyoshi Shuto, Mary Ann Suico, Yasuaki Hashimoto and Hirofumi Kai . DeltaF508 CFTR pool in the ER is increased by calnexin overexpression, Mol.Biol.Cell, 15,563-574,2004

橋本泰明，甲斐広文　
日本薬理学会雑誌 (JPS) 124巻 1号 2004年7月
「最近の話題」
神経変性疾患の万能薬開発に向けて　( 日薬 理誌 124, 61 (2004)

FUMIYOSHI OJIMA, TATSUO IDO, JUN HATAZAWA, MASATOSHI ITOH, HIROSHI SHINOHARA, TAKAYUKI ISHII, SHOICHI WATANUKI, SHINYA SEO, HIROFUMI KAI, KAZUO TAKAHAMA, YOSHITO NAKAGAWA AND TAKESHI MIYATA Mucociliary clearance and pulmonary epiihelial permeability of normal and acute bronchitic rabbits measured by three-dimensional PET and rectilinear scan after inhalation of 2-［18F］fluoro-2-deoxy-D-glucose, Journal of Applied Therapeuic Research, Vol.4, 11-20 (2003)

首藤　剛, Li Jian-Dong, 甲斐広文. インフルエンザ菌感染症と自然免疫, 分子呼吸器病, Vol.7, No.5, 67-69 (2003)

原田一恒, 沖米田　司, 首藤　剛, 甲斐広文. 嚢胞性線維症の創薬ターゲット分子, 医学のあゆみ, Vol.207, No.1, 90-95, (2003)

安東由喜雄, 甲斐広文. FAPの創薬ターゲット分子-FAPの病態解析と治療-, 医学のあゆみ, Vol.207, No.1, 78-82 (2003)

城野博史. NTHi感染による粘液過剰産生メカニズム-TGF-βシグナルのあらたな役割-, 医学のあゆみ, Vol.207, No.1, 73-77 (2003)

関　由行, メアリー・アン・スイコ, 甲斐広文. 転写因子MEF-遺伝子診断・癌治療への応用-, 医学のあゆみ, Vol.207, No.1, 41-45 (2003)

首藤　剛, 甲斐広文. 癌温熱療法増感薬の開発, 医学のあゆみ, Vol.207, No.1, 7-11 (2003)

甲斐広文. ターゲット分子と創薬-OVERVIEW-, 医学のあゆみ, Vol.207, No.1, 3-5 (2003)

沖米田 司，首藤 剛，甲斐 広文．　カルネキシンと嚢胞性線維症．臨床化学，2003，Vol.32, 147-153.

H. Jono, H. Xu, H. Kai, D. J Lim, Y. S Kim, X.-H. Feng, and J.-D. Li,　TGF-b-Smad signaling pathway negatively regulates nontypeable haemophilus　influenzae-induced MUC5AC Mucin transcription via MAPK　phosphatase-1-dependent inhibition of p38 MAPK. J.Biol.Chem. 278, 27811-27819 (2003)

Z. Lu, K. Kim, M. Suico, A. Uto, Y. Seki, T. Shuto, Y. Isohama, T. Miyata and H. Kai, ETS2 is involved in protein kinase C-activated expression of granulocyte-macrophage colony-stimulating factor in human non-small lung carcinoma cell line, A549. Biochem. Biophys. Res. Commun. 303, 190-195 (2003)

A.Imasato , C. Desbois-Mouthon, J.Han, H.Kai, A.C.B.Cato, S.Akira, J.-D.Li, Inhibition of p38 MAPK by Glucocorticoids via induction of MAPK phosphatase-1 enhances nontypeable Haemophilus influenzae-induced expression of Toll-like receptor 2. J.Biol.Chem. 277. 47444-47450 (2002)

H. Jono, T. Shuto, H. Xu, H. Kai, D. J Lim, J. R Gum, Y. S Kim, S. Yamaoka, X.-H. Feng, and J.-D. Li
TGF-b-Smad signaling pathway cooperates with NF-kB to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription J. Biol. Chem., 277, 45547-45557 (2002)

Y. Seki, M.-A. Suico, A. Uto, A. Hisatsune, T. Shuto, Y. Isohama, and H. Kai, The ETS transcription factor MEF is a candidate tumor suppressor gene on the X chromosome, Cancer Res 62: 6579-6586 (2002)

S. Kunimoto, W. Murofushi, H. Kai, Y. Ishida, A. Uchiyama, T. Kobayashi, S. Kobayashi, H. Murofushi, K. Murofushi-Murakami, Steryl Glucoside is a lipid mediator in stress-responsive signal transduction. Cell Structure Function,27, 157-162 (2002)

T. Okiyoneda, I. Wada, H. Jono, T. Shuto, K. Yoshitake, N. Nakano, S. Nagayama, K. Harada, Y. Isohama, T. Miyata, H. Kai
Calnexin Δ185-520 partially reverses the misprocessing of the ΔF508 cystic fibrosis transmembrane conductance regulator. FEBS letters, 526, 87-92 (2002)

Y. Ando, M. Nakamura, H. Kai, S. Katsuragi, H. Terazaki, T. Nozawa, T. Okuda, S. Misumi, N. Matsunaga, K. Hata, T. Tajiri, S. Shoji, T. Yamashita, K. Haraoka, K. Obayashi, K. Matsumoto, M. Ando, and M. Uchino, A novel localized amyloidosis associated with lactoferrin in the cornea. Lab. Invest. 82, 757-766, 2002

特願　2002-137944，発明の名称；アミロイドーシスの予防及び治療のための医薬，発明者：甲斐広文，安東由喜雄（2002）

Tajiri T, Ando Y, Hata K, Kamiide K, Hashimoto M, Nakamura M,Terazaki H, Yamashita T, Kai H, Haraoka K, Imasato A, Takechi K, Nakagawa K, Ishizaki T. Amyloid formation in rat transthyretin: effect of oxidative stress. Clin Chim Acta. 323(1-2):129-37 (2002).

M.-A. Suico, T. Koyanagi, S. Ise, Z. Lu, A. Hisatsune, Y. Seki, T. Shuto, Y.　Isohama, T. Miyata, and H. Kai, Functional dissection of the ETS transcription factor MEF, Biochem. Biochim. Acta, 1577, 113-120 (2002).

Okiyoneda T, Kai H. Introduction of the conventional method of DNA transfection by adenovirus vector. Nippon Yakurigaku Zasshi.119(6):337-44. (Japanese)(2002).

Shuto T, Imasato A, Jono H, Sakai A, Xu H, Watanabe T, Rixter DR, Kai H, Andalibi A, Linthicum F, Guan YL, Han J, Cato AC, Lim DJ, Akira S, Li JD. Glucocorticoids synergistically enhance nontypeable Haemophilus influenzae-induced Toll-like receptor 2 expression via a negative cross-talk with p38 MAP kinase. J Biol Chem. 2002 May 10;277(19):17263-17270.

甲斐広文，首藤　剛．分子シャペロンをターゲットにした創薬研究．ファルマシア，2002，Vol.38, 296-300.

H. Yano, F. Hirayama, M. Kamada, H. Arima, K. Uekama, Colon-specific delivery of prednisolone-appended a-cyclodextrin conjugate: alleviation of systemic side effect after oral administration. J. Control Release. 79, 103-112, 2002.

Y. Nagase, M. Hirata, H. Arima, S. Tajiri, Y. Nishimoto, F. Hirayama, T. Irie, K. Uekama, Novel protective effect of sulfobutyl ether b-cyclodextrin on DY-9760e-induced hemolysis to in vitro. J. Pharm. Sci., in press, 2002.

H. Arima, F. Hirayama, C.T. Okamoto and K. Uekama, Recent aspects of cyclodextrin-based pharmaceutical formulations. ﾒRecent Research Developments in Chemical & Pharmaceutical Sciences", Transworld Research Network, India, 2002, in press.

特願　2001-212354，発明の名称；熱ショック蛋白質の合成抑制及び機能抑制剤，出願人：甲斐広文（2001）

国内出願番号：特願2001-034986, 発明の名称: ETS転写因子又はそれをコードする遺伝子からなる上皮細胞のGM-CSF,及び／又はディフェンシン
蛋白質の発現調節剤,出願人：甲斐広文 (2001)

国内出願番号：特願2001-034834, 発明の名称: ETS転写因子又はそれをコードする遺伝子からなる細胞増殖抑制剤，出願人：甲斐広文(2001)

Nagayama S, Jono H, Suzaki H, Sakai K, Tsuruya E, Yamatsu I, Isohama Y, Miyata T, Kai H. 　Carbenoxolone, a new inducer of heat shock protein 70.　Life Sci. 2001;69(24):2867-73.

Komori T, Kai H, Shimoishi K, Kabu K, Nonaka A, Maruyama T, Tamura K, Otagiri M. 　Up-regulation by clarithromycin of alpha(1)-acid glycoprotein expression in liver and primary cultured hepatocytes.　Biochem Pharmacol. 2001;62(10):1391-7.

Shuto T, Xu H, Wang B, Han J, Kai H, Gu XX, Murphy TF, Lim DJ, Li JD. 　Activation of NF-kappa B by nontypeable Hemophilus influenzae is mediated by toll-like receptor 2-TAK1-dependent NIK-IKK alpha /beta-I kappa B alpha and MKK3/6-p38 MAP kinase signaling pathways in epithelial cells.　Proc Natl Acad Sci U S A. 2001;98(15):8774-9.

Takahashi K, Kai H, Mizuno H, Koda T, Miyata T. 　Effect of fudosteine, a new cysteine derivative, on mucociliary transport.
J Pharm Pharmacol. 2001;53(6):911-4.

Shimoishi K, Kai H, Kabu K, Komori T, Maruyama T, Otagiri M.　FK506 (tacrolimus) increases rat alpha1-acid glycoprotein expression in liver and primary cultured hepatocytes.　Eur J Pharmacol. 2001;420(2-3):91-5.

Isohama Y, Kanemaru M, Kai H, Takahama K, Miyata T. 　Interaction between beta-adrenergic signaling and protein kinase C increases cytoplasmic Ca2+ in alveolar type II cells.　Life Sci. 2001;68(21):2361-71.

N. Ono, F. Hirayama, H. Arima, K. Uekama, Analysis of the phase solubility diagram of a phenacetin/competitor/ b-cyclodextrin ternary system, involving competitive inclusion complexation. Chem. Pharm. Bull., 49, 78-81, 2001.

N. Ono, H. Arima, F. Hirayama and K. Uekama. A Moderate interaction of maltosyl-a-cyclodextrin with caco-2 cells in comparison with the parent cyclodextrin. Biol. Pharm. Bull., 24, 395-402, 2001.

H. Arima, K. Yumomae, K. Miyake, T. Irie, F. Hirayama and K. Uekama, Comparative studies of the enhancing effects of cyclodextrins on the solubility and oral bioavailability of tacrolimus in rats. J. Pharm. Sci., 90, 690-701, 2001.

H. Arima, K. Yunomae, F. Hirayama and K. Uekama, Contribution of P-glycoprotein to the enhancing effects of dimethyl-b-cyclodextrin on oral bioavailability of tacrolimus. J. Pharmacol. Exp. Ther., 297, 547-555, 2001.

H. Arima, F. Kihara, F. Hirayama and K. Uekama, Enhancement of gene expression by polyamidoamine dendrimer conjugates with a-, b- and g-cyclodextrins. Bioconjugate Chem., 12, 476-484, 2001.

H. Arima, Y. Nishimoto, K. Motoyama, F. Hirayama and K. Uekama, Inhibitory effects of novel hydrophilic cyclodextrin derivatives on nitric oxide production in macrophages stimulated with lipopolysaccharide. Pharm. Res., 18, 1167-1173, 2001.

H. Yano, F. Hirayama, H. Arima, K. Uekama, Prednisolone-appended a-cyclodextrin: alleviation of systemic adverse effect of prednisolone after intracolonic administration in 2,4,6-trinitrobenzenesulfonic acid-induced colitis rats. J. Pharm. Sci., 90, 493-503, 2001.

H. Yano, M. Kamada, F. Hirayama, H. Arima, K. Uekama, Prednisolone-appended a-cyclodextrin conjugate: drug release in rat biological fluids and anti-inflammatory effect after oral administration to 2,4,6-trinitrobenzenesulfonic acid-induced colitis rats, J. Pharm. Sci., 90, 2103-2112, 2001

K. Uekama, Y. Hieda, F. Hirayama, H. Arima, M. Sudoh, A. Yagi, and H. Terashima, The Stabilizing and solubilizing effects of sulphobutyl ether b-Cyclodextrin (SBE-b-CyD) on prostaglandin E1 analogue. Pharm. Res., 18, 1578-1585, 2001.

Y. Nagase, M. Hirata, K. Wada, H. Arima, F. Hirayama, T. Irie, M. Kikuchi, and K. Uekama, Interaction of DY-9760e, a Novel Cytoprotective agent with positive charge, with sulfobutyl ether β-cyclodextrin and 2-hydroxypropyl-b-cyclodextrin: Improvements of solubility, photo-stability and adsorption. Int. J. Pharm., 229, 163-172, 2001.

H. Matsuda, M. Yamaguchi and H. Arima, Separation and crystallization of oleaginous constituents in cosmetics: Sweating and blooming. ﾒCrystallization Processes in Fats and Lipid Systemsﾓ, Marcel Dekker, Inc., New York, pp. 485-503, 2001.

Cyclodextrin-dendrimer conjugates as gene transfection vector. Arima, Hidetoshi; Kihara, Fumihiro; Uegama, Kaneto. (Nihon Shokuhin Kako K. K., Japan). Jpn. Kokai Tokkyo Koho (2001), 10 pp. CODEN: JKXXAF JP 2001103969 A2 20010417 Patent written in Japanese. Application: JP 99-288792 19991008. CAN 134:276488 AN 2001:270155 CAPLUS

国内特許出願番号：特願平11-3444795，国際出願番号PCT/JP00/008529, 発明の名称：新規な小胞体局在タンパク質およびそれを含む糖タンパク質異常症治療薬，並びに 該タンパク質をコードするDNAおよびそれを含む糖タンパク質異常症治療のための遺伝子治療薬， 出願人：甲斐広文 (2000)

Takahashi K, Iwase N, Ishikawa M, Mizuno H, Koda T, Kai H, Miyata T. 　[Effects of fudosteine, a new cysteine derivative, on airway secretion in rabbits and rats]　Nippon Yakurigaku Zasshi. 2000;116(6):371-8.

Suzuki K, Shono F, Kai H, Uno T, Uyeda M. 　Inhibition of topoisomerases by fatty acids.　J Enzyme Inhib. 2000;15(4):357-66.

Okumura M, Kai H, Arimori K, Iwakiri T, Hidaka M, Shiramoto S, Isohama Y, Miyata T. 　Adrenomedullin increases phosphatidylcholine secretion in rat type II pneumocytes.　Eur J Pharmacol. 2000;403(3):189-94.

Y. Ikeda, K. Kimura, F. Hirayama, H. Arima and K. Uekama, Controlled release of a water-soluble drug, captopril, by a combination of hydrophilic and hydrophobic cyclodextrin derivatives. J. Control. Release, 66 , 271-280, 2000.

K. Kimura, F. Hirayama, H. Arima and K. Uekama, Selective crystallization of tolbuamide polymorphs controlled by inclusion complexation with amorphous 2-hydroxypropyl-a- and b-cyclodextrins. Chem. Pharm. Bull., 48 , 646-650, 2000.

Y. Aramaki, S. Takano, H. Arima and S. Tsuchiya. Induction of apoptosis in WEHI 231 cells by cationic liposomes. Pharm. Res., 17 , 515-520, 2000.

K. Miyake, H. Arima, F. Hirayama, M. Yamamoto, T. Horikawa, H. Sumiyoshi, S. Noda and K. Uekama, Improvement of solubility and oral bioavailability of rutin by complexation with 2-hydroxypropyl-b-cyclodextrin. Pharm. Develop. Tech., 5 , 399-407, 2000.

K. Tokihiro, H. Arima, S. Tajiri, T. Irie, F. Hirayama and K. Uekama, Improvement of subcutaneous bioavailability of insulin by sulfobutyl ether b-cyclodextrin in rats. J. Pharm. Pharmacol., 52 , 911-917, 2000.

F. Hirayama, M. Honjo, H. Arima, K. Okimoto and K. Uekama, Characterizatin of nilvadipine monohydrate: crystal structure and phase transition behavior. Eur. J. Pharm. Sci., 11 , 81-88, 2000.

H. Yano, F. Hirayama, H. Arima and K. Uekama, Hydrolysis behavior of predonisolone 21-hemisuccinate/b-cyclodextrin amide conjugate: Involvement of intramolecular catalysis of amide group in drug release. Chem. Pharm. Bull., 48 , 1125-1128, 2000.

F. Hirayama, T. Ogata, H. Yano, H. Arima, K. Udo, M. Takano and K. Uekama, Release characteristics of a short-chain fatty acid, n -butyric acid, from its b-cyclodextrin ester conjugate in rat biological media. J. Pharm. Sci., 89 , 1486-1495, 2000.

Hisatsune A, Uto A, Koyanagi T, Chihara T, Miyata T, Basbaum C, Kai H. 　[Novel transcription factor MEF is associated with the function of lung epithelial cells]　Nippon Yakurigaku Zasshi. 1999;114 Suppl 1:81P-85P.

Hosoe H, Kaise T, Ohmori K, Isohama Y, Kai H, Takahama K, Miyata T. 　Mucolytic and antitussive effects of erdosteine.
J Pharm Pharmacol. 1999;51(8):959-66.

Nagayama S, Kai H, Okiyoneda T, Horikawa S, Miyata T. 　Characterization of CFTR expression in a human pulmonary mucoepidermoid carcinoma cell line, NCI-H292 cells.　FEBS Lett. 1999;455(3):215-8.

Kai H, Hisatsune A, Chihara T, Uto A, Kokusho A, Miyata T, Basbaum C. 　Myeloid ELF-1-like factor up-regulates lysozyme transcription in epithelial cells.　J Biol Chem. 1999;274(29):20098-102.

Okumura M, Kai H, Shinozawa S, Isohama Y, Miyata T. 　Effects of eosinophil granule major basic protein on phosphatidylcholine secretion in rat type II pneumocytes.　Am J Physiol. 1999;276(5 Pt 1):L763-8.

Tai S, Kai H, Isohama Y, Moriuchi H, Hagino N, Miyata T. 　The effect of maimendongtang on airway clearance and secretion.
Phytother Res. 1999;13(2):124-27.

K. Miyake, H. Arima, T. Irie, F. Hirayama and K. Uekama, Enhanced absorption of cyclosporin A by complexation with dimethyl-b-cyclodextrin in bile duct-cannulated and ﾐnoncannulated rats. Biol. Pharm. Bull., 22 , 66-72, 1999.

K. Miyake, T. Irie, H. Arima, F. Hirayama, K. Uekama, M. Hirano, Y. Okamoto, Characterization of itraconazole/2-hydroxypropyl-b-cyclodextrin inclusion complex in aqueous propylene glycol solution. Int. J. Pharm., 179 , 237-245, 1999.

N. Ono, F. Hirayama, H. Arima and K. Uekama, Determination of stability constant of b-cyclodextrin complexes using the membrane permeation technique and the permeation behavior of drug-competing agent-b-cyclodextrin ternary systems. Eur. J. Pharm. Sci., 8 , 133-139, 1999.

Y. Morimura, Y. Aramaki, H. Arima, T. Sakamoto, L. Insugi and S. Tsuchiya, Resistance of plasmid DNA to DNase by complex formation with cationic liposomes. Drug Delivery system, 14 , 165-169, 1999.

Y. Hamano, K. Ito, H. Matsuda, H. Sumioshi, H. Arima, F. Hirayama and K. Uekama, Inhibitory effect of 2-hydroxypropyl-b-cyclodextrin on the foaming generated by the phosphodiester compound of vitamin C and E, EPC-K1. Chem. Pharm. Bull., 47 , 828-832, 1999.

F. Hirayama, S. Mieda, Y. Miyamoto, H. Arima and K. Uekama, Heptakis(2,6-di- O -methyl-3- O -acetyl)-b-cyclodextrin: A water-soluble cyclodextrin derivatives with low hemolytic activity. J. Pharm. Sci., 88 , 970-975, 1999.

H. Arima, M. Takahashi, Y. Aramaki, T. Sakamoto, K. Yuda, K. Akiyama, T. Goto and S. Tsuchiya, Design of potent phosphorothioate antisense oligonucleotides directed to human interleukin 10 gene product and their evaluation of antisense activity in U937 cells. Pharm. Res., 16 , 1163-1171, 1999.

K. Kimura, F. Hirayama, H. Arima and K. Uekama, Solid-state 13C nuclear magnetic resonance spectroscopic study on amorphous solid complexes of tolbutamide with 2-hydroxypropyl-a- and -b-cyclodextrin. Pharm. Res., 16 , 1729-1734, 1999.

Y. Aramaki, R. Matsuno, H. Arima and S. Tsuchiya, Macrosialin may not be involved with the regulation of nitric oxide production from mouse peritoneal macrophages stimulated with LPS. Res. Commun. Molec. Pathol. Pharmacol. 104 , 22-30, 1999.

H. Matsuda and H. Arima, Cyclodextrins in transdermal and Rectal Delivery. Adv. Drug Deliv. Reviews, 36 , 81-99, 1999.

Miyata T, Isohama Y, Kai H. [Exploration on mechanism of Chinese medical recipe for cough-remedy and sputum-removing]
Zhongguo Zhong Xi Yi Jie He Za Zhi. 1998;18(8):502-3.

Ishii T, Kibushi N, Nakajima T, Kakuta T, Tanaka N, Sato C, Sugai K, Kijima-Suda I, Kai H, Miyata T. Local anti-inflammatory activity and systemic side effects of NM-135, a new prodrug glucocorticoid, in an experimental inflammatory rat model.Jpn J Pharmacol. 1998;78(4):505-9.

Ojima F, Ido T, Hatazawa J, Itoh M, Shinohara H, Watanuki S, Seo S, Kai H, Takahama K, Ishii T, Nakagawa Y, Miyata T.
Development of a new method for simultaneously evaluating mucociliary clearance and pulmonary epithelial permeability in rabbit experiments by means of 18FDG, three-dimensional positron emission tomography and rectilinear scan.Ann Nucl Med. 1998;12(5):231-5.

Takahashi K, Mizuno H, Ohno H, Kai H, Isohama Y, Takahama K, Nagaoka S, Miyata T. Effects of SS320A, a new cysteine derivative, on the change in the number of goblet cells induced by isoproterenol in rat tracheal epithelium.Jpn J Pharmacol. 1998;77(1):71-7.

Fukushima H, Nagayama S, Otsuka M, Takahama K, Isohama Y, Kai H, Miyata T. Inhibition of glycine-induced current by morphine in nucleus tractus solitarii neurones of guinea pigs.Methods Find Exp Clin Pharmacol. 1998;20(2):125-32.

Miyata T, Kai H, Isohama Y, Takahama K. Current opinion of muco-active drug research: strategies and problems.
Eur Respir J. 1998;11(2):480-91. Review.

H. Arima, T. Miyaji, T. Irie, F. Hirayama and K. Uekama, Enhancing effect of hydroxypropyl-b-cyclodextrin on cutaneous penetration and activation of ethyl 4-biphenyl acetate in hairless mouse skin. Eur. J. Pharm. Sci., 6 , 53-59, 1998.

R. Matsuno, Y. Aramaki, H. Arima, Y. Adachi, N. Ohno, T. Yadomae and S. Tsuchiya, Contribution of CR3 to nitric oxide production from macrophages stimulated with high-dose of LPS. Biochem. Biophys. Res. Commun., 244 , 115-119, 1998.

H. Arima, M. Takahashi, Y. Aramaki and S. Tsuchiya, Specific inhibition of IL-10 production in murine macrophage-like cells by phosphorothioate antisense oligonucleotides. Antisense Nucl. Acid Drug. Develop., 8 , 319-327, 1998.